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Literature summary extracted from

  • Lakaye, B.; Makarchikov, A.F.; Antunes, A.F.; Zorzi, W.; Coumans, B.; De Pauw, E.; Wins, P.; Grisar, T.; Bettendorff, L.
    Molecular characterization of a specific thiamine triphosphatase widely expressed in mammalian tissues (2002), J. Biol. Chem., 277, 13771-13777.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.28 overexpression in Escherichia coli as a glutathione S-transferase fusion protein Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.28 5,5'-dithiobis-2-nitrobenzoic acid
-
 Bos taurus
3.6.1.28 5,5'-dithiobis-2-nitrobenzoic acid
-
 Homo sapiens
3.6.1.28 PCMB
-
 Bos taurus
3.6.1.28 PCMB
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.28 0.039
-
 thiamine triphosphate
-
 Bos taurus
3.6.1.28 0.126
-
 thiamine triphosphate
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.1.28 soluble
-
 Bos taurus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.28 23892
-
 1 * 23892, mass spectrometry Homo sapiens
3.6.1.28 23892
-
 1 * 23892, mass spectrometry Bos taurus
3.6.1.28 25000
-
 gel filtration Homo sapiens
3.6.1.28 25000
-
 gel filtration Bos taurus
3.6.1.28 27000
-
 1 * 27000, SDS-PAGE Homo sapiens
3.6.1.28 27000
-
 1 * 27000, SDS-PAGE Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.28 Bos taurus Q8MKF1 calf
-
3.6.1.28 Homo sapiens Q9BU02
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.28
-
 Homo sapiens
3.6.1.28
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.1.28 bladder
-
 Homo sapiens
-
3.6.1.28 brain
-
 Bos taurus
-
3.6.1.28 brain low activity Homo sapiens
-
3.6.1.28 kidney
-
 Homo sapiens
-
3.6.1.28 lung
-
 Homo sapiens
-
3.6.1.28 prostate high activity Homo sapiens
-
3.6.1.28 testis high activity Homo sapiens
-
3.6.1.28 thyroid gland
-
 Homo sapiens
-
3.6.1.28 uterus high activity Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.28 103
-
-
 Homo sapiens
3.6.1.28 103
-
-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.28 thiamine triphosphate + H2O
-
 Homo sapiens thiamine diphosphate + phosphate
-
 ?
3.6.1.28 thiamine triphosphate + H2O
-
 Bos taurus thiamine diphosphate + phosphate
-
 ?
3.6.1.28 thiamine triphosphate + H2O absolute specificity Homo sapiens thiamine diphosphate + phosphate
-
 ?
3.6.1.28 thiamine triphosphate + H2O absolute specificity Bos taurus thiamine diphosphate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.28 monomer 1 * 27000, SDS-PAGE Homo sapiens
3.6.1.28 monomer 1 * 27000, SDS-PAGE Bos taurus
3.6.1.28 monomer 1 * 23892, mass spectrometry Homo sapiens
3.6.1.28 monomer 1 * 23892, mass spectrometry Bos taurus

Synonyms

EC Number Synonyms Comment Organism
3.6.1.28 ThTPase
-
 Homo sapiens
3.6.1.28 ThTPase
-
 Bos taurus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.28 240
-
 thiamine triphosphate
-
 Homo sapiens
3.6.1.28 240
-
 thiamine triphosphate
-
 Bos taurus